Knockdown of VAMP8 attenuates atherosclerosis and enhances the effect of simvastatin in APOE-deficient mice

Purpose: To investigate the regulatory effects of vesicle-associated membrane protein 8 (VAMP8) in atherosclerosis (AS).
 Methods: VAMP8 expression was assessed using quantitative real time-polymerase chain reaction (qRT-PCR) and western blot, while H&E staining used to examine morphology arterial glandular tissues AS mice. Lipid accumulation mice determined with aid Oil Red O staining, whereas apoptosis aortic cells evaluated by TUNEL assay.
 Results: highly expressed advanced-stage samples, also elevated (p < 0.01). level rose Moreover, aorta showed atherosclerotic lesions intima thickening plaques group However, these changes were alleviated VAMP8-silenced silencing decreased lipid inflammation oxidative stress It observed that depletion reduced cell 0.05). Furthermore, knockdown enhanced effect simvastatin on 
 Conclusion: Knockdown alleviates ApoE-deficient This finding suggests this might be a potential strategy for prevention treatment AS.